592544-81-5
浏览量：28 ｜ 2024/4/30 19:23:24
FRET底物、FRET Substrates
编号：121540
CAS号：592544-81-5
三字母：MCA-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-DArg-NH2
描 述：Highly sensitive and selective cathepsin E FRET substrate derived from the cleavage site sequence of human α2-macroglobulin (Km = 1.9 µM; kcat/Km = 10.2 µM⁻¹s⁻¹ for human erythrocyte cathepsin E). The substrate was resistent to hydrolysis by the analogous aspartic proteinases cathepsin D and pepsin, as well as the lysosomal cysteine proteinases cathepsin B, L, and H. Useful for monitoring and accurately quantifying cathepsin E, even in crude enzyme preparations (excitation wavelength at 328 nm, emission wavelength at 393 nm).
高灵敏度和选择性的组织蛋白酶E FRET底物来源于人α2-巨球蛋白的裂解位点序列(Km = 1.9µM;(kcat/Km = 10.2 μ M)(人红细胞组织蛋白酶E)。底物可以抵抗类似的天冬氨酸蛋白酶组织蛋白酶D和胃蛋白酶以及溶酶体半胱氨酸蛋白酶组织蛋白酶B、L和h的水解。即使在粗酶制剂中(激发波长为328 nm，发射波长为393 nm)，也可以用于监测和准确定量组织蛋白酶E。
